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  • 欢迎选择我们,上海恒远生物科技有限公司!免费服务热线:400-0899-208          防伪查询

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    ACTA1 Antibody

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    ACTA1 Antibody

      恒远生物抗体平台现已拥有4万多种抗体(含单抗和多抗),包括人类常用抗体,模式生物类相关抗体、小分子抗体、二抗、标签抗体及内参抗体等。并以每年4000种的速度在持续增长。

      恒远生物拥有专业的质控团队,配备先进实验仪器,现已成功地建立了ChIP平台,因此我们的抗体不仅能提供Biotin、FITC、HRP等标记,大部分抗体都能进行ELISA、WB、IHC、IF、IP、ChIP等应用验证。

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    ACTA1 Antibody

     
    • 货号:
      HYK-200292
    • 规格:
       
    • 图片:
      • Western Blot
        Positive WB detected in:Hela whole cell lysate,A549 whole cell lysate,Raw264.7 whole cell lysate,NIH/3T3 whole cell lysate,HepG2 whole cell lysate,Rat brain tissue,Rat heart tissue
        All lanes:Actin antibody at 0.95μg/ml
        Secondary
        Goat polyclonal to rabbit IgG at 1/50000 dilution
        Predicted band size: 42 KDa
        Observed band size: 42 KDa
      • IHC image of CSB-RA001205A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
      • Immunofluorescence staining of HepG2 cells with CSB-RA001205A0HU at 1:60,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

    产品详情

    • Uniprot No.:
      P68133
    • 基因名:
      ACTA1
    • 别名:
      a actin antibody; ACTA antibody; ACTA1 antibody; ACTA2 antibody; ACTC antibody; ACTC1 antibody; Actin antibody; ACTS_HUMAN antibody; ACTSA antibody; Alpha 2 actin antibody; alpha skeletal muscle antibody; Alpha-actin-1 antibody; Cardiac muscle alpha actin 1 antibody; Skeletal muscle alpha actin 1 antibody
    • 反应种属:
      Human
    • 免疫原:
      A synthesized peptide
    • 免疫原种属:
      Homo sapiens (Human)
    • 标记方式:
      Non-conjugated
    • 克隆类型:
      Monoclonal
    • 抗体亚型:
      Rabbit IgG
    • 纯化方式:
      Affinity-chromatography
    • 浓度:
      It differs from different batches. Please contact us to confirm it.
    • 保存缓冲液:
      Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
    • 产品提供形式:
      Liquid
    • 应用范围:
      ELISA, WB, IHC, IF, FC
    • 推荐稀释比:
      Application Recommended Dilution
      WB 1:500-1:5000
      IHC 1:50-1:500
      IF 1:30-1:200
    • Protocols:
      ELISA Protocol
      Western Blotting (WB) Protocol
      Immunohistochemistry (IHC) Protocol
      Immunofluorescence (IF) Protocol
      Flow cytometry (FC) Protocol
    • 储存条件:
      Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
    • 货期:
      Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.


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